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准确测定有机化合物的分子结构，对从分子水平去认识物质世界，推动近代有机化学的发展是十分重要的。采用现代仪器分析方法，可以快速、准确地测定有机化合物的分子结构。在有机化学中应用最广泛的测定分子结构的方法是四大光谱法：紫外光谱、红外光谱、核磁共振和质谱。紫外和可见光谱（ultraviolet and visible spectrum）简写为UV。

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